Determination of l-ascorbic acid in human serum by chemiluminescence based on hydrogen peroxide–sodium hydrogen carbonate–CdSe/CdS quantum dots system

In the present work, a strongly chemiluminescence (CL) from the mixing of hydrogen peroxide (H 2O 2), hydrogen carbonate (HCO 3 −) and CdSe/CdS quantum dots (QDs) was observed. The addition of trace amount of l-ascorbic acid into the CL system caused significant quenching luminescence intensity. Based on this phenomenon, a flow injection CL analysis system for the determination of l-ascorbic acid was developed. The CL reaction conditions, such as mixing orders and concentrations of CL reagents, flow rates, pH, and particle size of quantum dots have been optimized. The CL intensity and the concentration of l-ascorbic acid have a good linear relationship in the ranges of 1.0 × 10 −7–1.0 × 10 −4 mol L −1 ( R 2 = 0.9916). The limit of detection for l-ascorbic acid is 6.7 × 10 −9 mol L −1 (S/N = 3). This method has been successfully applied to determine l-ascorbic acid in human serum. The recoveries for the real samples were from 83% to 111% and the variance coefficient was