Lactate Biosensor Based on Hydrotalcite-Like Compounds: Performances and Application to Serum Samples

A lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 μM and a sensitivity of 91 mA M−1 cm−...

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Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2009-11, Vol.21 (22), p.2401-2409
Hauptverfasser: Carpani, Irene, Guadagnini, Lorella, Tonelli, Domenica
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Sprache:eng
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Zusammenfassung:A lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 μM and a sensitivity of 91 mA M−1 cm−2, was obtained. The influence of some interferents due to the oxidation of hydrogen peroxide (at +0.35 V vs. SCE) was also studied. By controlling carefully the experimental conditions, the determination of lactate in a commercial serum sample in the presence of interferents was successfully accomplished.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200900118