Vaginal Cornification of the Mouse produced by Œstrogens in vitro

SEVERAL workers have successfully grown parts of the genital tract in tissue culture, but only a few studies have been concerned with the action of œstrogens under these conditions. Gaillard 1 was able to stimulate the uterus and seminal vesicles with œstrone, whereas Emmens and Ludford 2 failed to induce keratinization in explants of rat vagina with œstradiol-3 : 17β. Recently, we have successfully cultured the vagina of the mouse and produced keratinization by the addition of œstrogens to the culture medium.