Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase

Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase

A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separat...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2010-01, Vol.396 (2), p.775-781
Hauptverfasser: Chitta, K. R, Van Meter, D. S, Stalcup, A. M
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Biochemistry
Bromides
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
Food Science
High performance liquid chromatography
Ionic liquids
Laboratory Medicine
Leucine
Mathematical analysis
Methionine
Monitoring/Environmental Analysis
Original Paper
Other chromatographic methods
Peptides
Peptides - chemistry
Peptides - isolation & purification
Physiological aspects
Properties
Resins, Synthetic - chemical synthesis
Resins, Synthetic - chemistry
Separation
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Zusammenfassung:A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-009-3228-4