Studies on the purification of acid prostatic phosphatase

Studies on the purification of acid prostatic phosphatase

1. 1. Characteristics are given for facilitating 5000-fold purification of prostatic acid phosphatase. 2. 2. A purification starting with 40 human glands is described. 3. 3. Foaming as a purification technique is used and described. A relatively great purification is obtained with foaming. 4. 4. Exp...

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Veröffentlicht in:Archives of biochemistry and biophysics 1953-09, Vol.46 (1), p.141-153
Hauptverfasser: London, Morris, Hudson, Perry B.
Format: Artikel
Sprache:eng
Schlagworte:
Acid Phosphatase
Humans
Male
Old Medline
Phosphoric Monoester Hydrolases
Prostate - metabolism
Protein Tyrosine Phosphatases
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Zusammenfassung:1. 1. Characteristics are given for facilitating 5000-fold purification of prostatic acid phosphatase. 2. 2. A purification starting with 40 human glands is described. 3. 3. Foaming as a purification technique is used and described. A relatively great purification is obtained with foaming. 4. 4. Experiments designed to demonstrate surface inactivation are presented. A study of methods of minimizing denaturation due to glass surface was made, and methods compatible with assay and isolation are presented.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(53)90177-5