Influence of Cations on the Ninhydrin Reaction for the Determination of Amino-Acids

Influence of Cations on the Ninhydrin Reaction for the Determination of Amino-Acids

THE micro-determination of α-amino-acids with ninhydrin in n -butanol is highly sensitive, but often inaccurate due to the retardation of colour formation and the production of various red or blue colours instead of the purple compound usually obtained. It was found that traces of cations had a mark...

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Veröffentlicht in:Nature (London) 1953-09, Vol.172 (4377), p.543-543
Hauptverfasser: MEYER, HANAN, RIKLIS, EMANUEL
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - analysis
Cations
Humanities and Social Sciences
Leadership
letter
multidisciplinary
Ninhydrin
Old Medline
Science
Science (multidisciplinary)
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Zusammenfassung:THE micro-determination of α-amino-acids with ninhydrin in n -butanol is highly sensitive, but often inaccurate due to the retardation of colour formation and the production of various red or blue colours instead of the purple compound usually obtained. It was found that traces of cations had a marked influence on the speed of colour formation and its intensity and on the absorption spectra of the coloured compounds produced. (The effect of cations on the ninhydrin colour was employed for the preservation of chromatogram spots by Kawerau and Wieland 1 .)
ISSN:0028-0836
1476-4687
DOI:10.1038/172543a0