The use of 125I-labelled protein a for the detection of humoral immunity to gross murine leukaemia virus

A solid-phase radioimmunoassay utilising binding of 125I-labelled protein A to antibodies bound to virus adsorbed onto microtitre plates was shown to be suitable for detection of humoral immunity to Gross murine leukaemia virus (MuLV). The specificity of the reaction was shown by the fact that only...

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Veröffentlicht in:	Journal of immunological methods 1980, Vol.36 (3), p.235-241
Hauptverfasser:	Holmes, H.C., Tuffrey, Maureen, Steuden, Jadwiga, Hilgers, J., Barnes, R.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	AKR murine leukemia virus - immunology Animals Antibody Formation Antigen-Antibody Reactions Antigens, Viral - analysis Iodine Radioisotopes Leukemia Virus, Murine - immunology Mice Mice, Inbred AKR Mice, Inbred CBA Precipitin Tests Radioimmunoassay - methods Staphylococcal Protein A - immunology
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Beschreibung
Zusammenfassung:	A solid-phase radioimmunoassay utilising binding of 125I-labelled protein A to antibodies bound to virus adsorbed onto microtitre plates was shown to be suitable for detection of humoral immunity to Gross murine leukaemia virus (MuLV). The specificity of the reaction was shown by the fact that only homologous or closely related viruses effectively inhibited binding of antibodies to adsorbed virus. With this method a low level of spontaneous humoral immunity was demonstrated in sera from AKR/Crc mice, a strain with high concentrations of endogenous virus, whereas little or no anti-viral activity was found in CBA/H-T6Crc, a subline that does not appear to express MuLV.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(80)90129-5