Regulation of glucose-6-P dehydrogenase activity in primary rat hepatocyte cultures

The glucose-6-P dehydrogenase specific activity in rat hepatocytes increases approximately 10-fold when the cells are placed into culture for three days. The induction requires insulin with maximum enzyme levels occurring at 10 −7 M. Pulse-labeling experiments revealed a 10-fold increase in the enzy...

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Veröffentlicht in:	Biochemical and biophysical research communications 1980-01, Vol.96 (2), p.748-755
Hauptverfasser:	Winberry, Larry, Nakayama, Roderick, Wolfe, Ronald, Holten, Darold
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cells, Cultured Enzyme Induction Glucosephosphate Dehydrogenase - biosynthesis Hydrocortisone - pharmacology Insulin - pharmacology Liver - cytology Liver - enzymology Male Rats Triiodothyronine - pharmacology
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Zusammenfassung:	The glucose-6-P dehydrogenase specific activity in rat hepatocytes increases approximately 10-fold when the cells are placed into culture for three days. The induction requires insulin with maximum enzyme levels occurring at 10 −7 M. Pulse-labeling experiments revealed a 10-fold increase in the enzyme's relative rate of synthesis after only 8 hours in culture.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(80)91418-7