CHARACTERIZATION OF AROMATIC HEPTAENE MACROLIDE ANTIBIOTICS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

CHARACTERIZATION OF AROMATIC HEPTAENE MACROLIDE ANTIBIOTICS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

High performance liquid chromatographic (HPLC) procedures were utilized for the rapid and efficient separation and characterization of the aromatic heptaene macrolide group of antifungal antibiotics. The instrument utilized a 350nm phosphor converted ultraviolet detector and a μ Bondapak C18 column...

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Veröffentlicht in:Journal of antibiotics 1980, Vol.33(6), pp.591-599
Hauptverfasser: MECHLINSKI, W., SCHAFFNER, C. P.
Format: Artikel
Sprache:eng
Schlagworte:
Antifungal Agents - analysis
Candicidin - analysis
Chromatography, High Pressure Liquid - methods
Organic Chemicals
Polyenes - analysis
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Zusammenfassung:High performance liquid chromatographic (HPLC) procedures were utilized for the rapid and efficient separation and characterization of the aromatic heptaene macrolide group of antifungal antibiotics. The instrument utilized a 350nm phosphor converted ultraviolet detector and a μ Bondapak C18 column packing. Optimum resolution of eleven commercial aromatic heptaene macrolide preparations was obtained with solvent systems consisting of mixtures of acetonitrile and 0.05 M aqueous sodium citrate buffer, pH 5.3. The presence of two distinct types of aromatic heptaene macrolides with numerous well-defined individual components has been established.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.33.591