Localization of Glycolytic and Respiratory Enzyme Systems on Isolated Mouse Brain Mitochondria.

Summary 1. Washed mouse brain mitochondria carried out the complete enzymic conversion of exogenous glucose to carbon dioxide and water. Glucolysis took place an-aerobically and aerobically, with the production of lactic acid. Under aerobic conditions glucose was oxidized by means of a malonate-sensitive cycle. The accumulation of lactic acid was correspondingly decreased. 2. In contrast, the supernatant carried out anaerobic and aerobic glucolysis, but not respiration of glucose. The submicroscopic particle fraction did not carry out the reactions of either system.