The effect of substituents on the hydrolysis of phenyl acetate by wheat germ lipase

The effect of substituents on the hydrolysis of phenyl acetate by wheat germ lipase

1. 1. Substituted phenyl acetates have been studied as substrates for wheat germ lipase. 2. 2. The enzymatically catalyzed hydrolysis of these substrates follows the usual Michaelis-Menten formulation. 3. 3. A mechanism for the enzymatic hydrolysis is proposed, and observed k 3 E 0 ( Vmax) values an...

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Veröffentlicht in:Archives of biochemistry and biophysics 1953-06, Vol.44 (2), p.455-467
Hauptverfasser: Gawron, Oscar, Grelecki, Chester J., Duggan, Marjorie
Format: Artikel
Sprache:eng
Schlagworte:
Acetates - metabolism
Hydrolysis
Lipase
Old Medline
Phenols
Plant Oils
Triticum
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Zusammenfassung:1. 1. Substituted phenyl acetates have been studied as substrates for wheat germ lipase. 2. 2. The enzymatically catalyzed hydrolysis of these substrates follows the usual Michaelis-Menten formulation. 3. 3. A mechanism for the enzymatic hydrolysis is proposed, and observed k 3 E 0 ( Vmax) values and calculated k 1 E 0 values are correlated with this mechanism.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(53)90063-0