Crystallization and preliminary X-ray investigation of calmodulin

Crystals of rat testis calmodulin, a multifunctional Ca2+-binding protein have been grown from solutions of 2-methyl-2,4-pentanediol. The crystals are triclinic, space group P1, with a = 29.79(4) A, b = 53.74(7) A, c = 24.78(3) A, alpha = 93.46(2)degrees, beta = 96.98(2)degrees, and gamma = 89.05(3)...

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Veröffentlicht in:	The Journal of biological chemistry 1980-09, Vol.255 (17), p.8152-8153
Hauptverfasser:	Cook, W J, Dedman, J R, Means, A R, Bugg, C E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Calcium-Binding Proteins - isolation & purification Calmodulin - isolation & purification Crystallization Male Protein Conformation Rats Testis - analysis X-Ray Diffraction
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Crystals of rat testis calmodulin, a multifunctional Ca2+-binding protein have been grown from solutions of 2-methyl-2,4-pentanediol. The crystals are triclinic, space group P1, with a = 29.79(4) A, b = 53.74(7) A, c = 24.78(3) A, alpha = 93.46(2)degrees, beta = 96.98(2)degrees, and gamma = 89.05(3)degrees. There is 1 calmodulin molecule per unit cell. The crystals are quite stable to x-rays and diffract beyond 2.5 A resolution.
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(19)70621-8