A calibration procedure for the determination of alkaline phosphatase with the autoanalyzer
We describe a kinetic procedure using the LKB reaction rate analyser for the calibration of an AutoAnalyzer alkaline phosphatase method. Measurements were made at zero incubation time with a special lid for the thermostated tunnel of the LKB reaction rate analyser using assay conditions appropriate...
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| Veröffentlicht in: | Clinica chimica acta 1980-01, Vol.105 (3), p.361-366 |
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| container_title | Clinica chimica acta |
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| creator | Nisbet, Jennifer A. Cleeve, H.J.W. Tua, Diane |
| description | We describe a kinetic procedure using the LKB reaction rate analyser for the calibration of an AutoAnalyzer alkaline phosphatase method. Measurements were made at zero incubation time with a special lid for the thermostated tunnel of the LKB reaction rate analyser using assay conditions appropriate to the AutoAnalyzer method. The molar absorptivity of
p-nitrophenol was estimated on the LKB reaction rate analyser used for the assay of the enzyme standards. Other variables affecting the accuracy of the measurement were also determined. |
| doi_str_mv | 10.1016/0009-8981(80)90116-3 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1980-01, Vol.105 (3), p.361-366 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75231174 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alkaline Phosphatase - metabolism Autoanalysis - standards Calibration - standards Clinical Enzyme Tests - instrumentation Humans Kinetics |
| title | A calibration procedure for the determination of alkaline phosphatase with the autoanalyzer |
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