A calibration procedure for the determination of alkaline phosphatase with the autoanalyzer

We describe a kinetic procedure using the LKB reaction rate analyser for the calibration of an AutoAnalyzer alkaline phosphatase method. Measurements were made at zero incubation time with a special lid for the thermostated tunnel of the LKB reaction rate analyser using assay conditions appropriate to the AutoAnalyzer method. The molar absorptivity of p-nitrophenol was estimated on the LKB reaction rate analyser used for the assay of the enzyme standards. Other variables affecting the accuracy of the measurement were also determined.