Use of chromatography tubes in triiodothyronine and thyroxine RIA

Use of chromatography tubes in triiodothyronine and thyroxine RIA

Simple, convenient radioimmunoassays for total triiodothyronine and total thyroxine in human serum are described. Ascending dry column chromatography is used to separate the free and antibody-bound hormone. The method allows for separation by absorption, without centrifugation. The use of test tubes...

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Veröffentlicht in:Clinica chimica acta 1980-01, Vol.106 (2), p.129-133
Hauptverfasser: Inbar, D., Tamir, Y., Derfler, Sara, Feingers, Judith, Wagner, D.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chromatography, Gel
Rabbits
Radioimmunoassay - methods
Thyroxine - blood
Triiodothyronine - blood
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Zusammenfassung:Simple, convenient radioimmunoassays for total triiodothyronine and total thyroxine in human serum are described. Ascending dry column chromatography is used to separate the free and antibody-bound hormone. The method allows for separation by absorption, without centrifugation. The use of test tubes containing pre-measured quantities of lyophilized radioactive reagent obviates unnecessary handling of radioactive solutions.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(80)90165-5