An Assessment of the Corning Electrophoretic Procedure for High Density Lipoprotein Cholesterol Estimation
The electrophoretic procedure for high density lipoprotein cholesterol determination recently introduced by Corning Medical has been assessed. The results correlated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the c...
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| Veröffentlicht in: | Annals of clinical biochemistry 1980-05, Vol.17 (3), p.148-152 |
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| container_title | Annals of clinical biochemistry |
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| creator | Bullock, D G Carter, T J N Cosgrove, Carmel M Hughes, Susan V |
| description | The electrophoretic procedure for high density lipoprotein cholesterol determination recently introduced by Corning Medical has been assessed. The results correlated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the clinically important range below 1 mmol/l. The densitometer, which must be carefully aligned before use for this analysis, seemed sensitive to variations in the plates and patterns produced. The technique required great care, especially with timing, and appeared expensive. |
| doi_str_mv | 10.1177/000456328001700309 |
| format | Article |
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The results correlated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the clinically important range below 1 mmol/l. The densitometer, which must be carefully aligned before use for this analysis, seemed sensitive to variations in the plates and patterns produced. The technique required great care, especially with timing, and appeared expensive.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/000456328001700309</identifier><identifier>PMID: 7406438</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Chemical Precipitation ; Cholesterol - blood ; Densitometry ; Electrophoresis, Agar Gel - methods ; Humans ; Lipoproteins, HDL - blood ; Reagent Kits, Diagnostic - standards ; Ultracentrifugation</subject><ispartof>Annals of clinical biochemistry, 1980-05, Vol.17 (3), p.148-152</ispartof><rights>1980 Association for Clinical Biochemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-79ea50f2b42434caf32727c8ea60b047ac00b79cb0f4a34ef04599c13f6bfe993</citedby><cites>FETCH-LOGICAL-c382t-79ea50f2b42434caf32727c8ea60b047ac00b79cb0f4a34ef04599c13f6bfe993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7406438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bullock, D G</creatorcontrib><creatorcontrib>Carter, T J N</creatorcontrib><creatorcontrib>Cosgrove, Carmel M</creatorcontrib><creatorcontrib>Hughes, Susan V</creatorcontrib><title>An Assessment of the Corning Electrophoretic Procedure for High Density Lipoprotein Cholesterol Estimation</title><title>Annals of clinical biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>The electrophoretic procedure for high density lipoprotein cholesterol determination recently introduced by Corning Medical has been assessed. The results correlated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the clinically important range below 1 mmol/l. The densitometer, which must be carefully aligned before use for this analysis, seemed sensitive to variations in the plates and patterns produced. The technique required great care, especially with timing, and appeared expensive.</description><subject>Chemical Precipitation</subject><subject>Cholesterol - blood</subject><subject>Densitometry</subject><subject>Electrophoresis, Agar Gel - methods</subject><subject>Humans</subject><subject>Lipoproteins, HDL - blood</subject><subject>Reagent Kits, Diagnostic - standards</subject><subject>Ultracentrifugation</subject><issn>0004-5632</issn><issn>1758-1001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotX78AUHIydvqbJJudo-l1g8o6EHPSzadtCnbpCbZg__elBYvgqdhZp55eecl5KaE-7KU8gEAxKTirAYoJQCH5oSMSzmpizJPTsl4DxR74pxcxLjJLcvciIykgErwekw2U0enMWKMW3SJekPTGunMB2fdis571Cn43doHTFbT9-A1LoeA1PhAX-xqTR_RRZu-6cLu_C74hNbR2dr3GBMG39N5THarkvXuipwZ1Ue8PtZL8vk0_5i9FIu359fZdFFoXrNUyAbVBAzrBBNcaGU4k0zqGlUFHQipNEAnG92BEYoLNDmDptElN1VnsGn4Jbk76GY7X0P20W5t1Nj3yqEfYisnjFWcswyyA6iDjzGgaXchew3fbQntPuD2b8D56PaoPnRbXP6eHBPN-4fDPqoVths_BJef_U_xB3K1hDY</recordid><startdate>198005</startdate><enddate>198005</enddate><creator>Bullock, D G</creator><creator>Carter, T J N</creator><creator>Cosgrove, Carmel M</creator><creator>Hughes, Susan V</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198005</creationdate><title>An Assessment of the Corning Electrophoretic Procedure for High Density Lipoprotein Cholesterol Estimation</title><author>Bullock, D G ; Carter, T J N ; Cosgrove, Carmel M ; Hughes, Susan V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-79ea50f2b42434caf32727c8ea60b047ac00b79cb0f4a34ef04599c13f6bfe993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Chemical Precipitation</topic><topic>Cholesterol - blood</topic><topic>Densitometry</topic><topic>Electrophoresis, Agar Gel - methods</topic><topic>Humans</topic><topic>Lipoproteins, HDL - blood</topic><topic>Reagent Kits, Diagnostic - standards</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bullock, D G</creatorcontrib><creatorcontrib>Carter, T J N</creatorcontrib><creatorcontrib>Cosgrove, Carmel M</creatorcontrib><creatorcontrib>Hughes, Susan V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bullock, D G</au><au>Carter, T J N</au><au>Cosgrove, Carmel M</au><au>Hughes, Susan V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Assessment of the Corning Electrophoretic Procedure for High Density Lipoprotein Cholesterol Estimation</atitle><jtitle>Annals of clinical biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>1980-05</date><risdate>1980</risdate><volume>17</volume><issue>3</issue><spage>148</spage><epage>152</epage><pages>148-152</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><abstract>The electrophoretic procedure for high density lipoprotein cholesterol determination recently introduced by Corning Medical has been assessed. The results correlated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the clinically important range below 1 mmol/l. The densitometer, which must be carefully aligned before use for this analysis, seemed sensitive to variations in the plates and patterns produced. The technique required great care, especially with timing, and appeared expensive.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>7406438</pmid><doi>10.1177/000456328001700309</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0004-5632 |
| ispartof | Annals of clinical biochemistry, 1980-05, Vol.17 (3), p.148-152 |
| issn | 0004-5632 1758-1001 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Chemical Precipitation Cholesterol - blood Densitometry Electrophoresis, Agar Gel - methods Humans Lipoproteins, HDL - blood Reagent Kits, Diagnostic - standards Ultracentrifugation |
| title | An Assessment of the Corning Electrophoretic Procedure for High Density Lipoprotein Cholesterol Estimation |
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