Transitional states of the neuronal serotonergic site

Binding of [ 3H]5-HT was studied wuing purified neuronal membranes isolted frrom horse brain striatum. It is shown that the preincubation of these membranes with an agonist (5-HT, bufotenin) followed by washibng, modifined the binding of [ 3H]5-GT from a low affinity to a high affinity constant. Thi...

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Veröffentlicht in:	European journal of pharmacology 1980-01, Vol.65 (1), p.109-112
Hauptverfasser:	Fillion, Gilles, Fillion, Marie-Paule
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Hydroxytryptamine receptor Animals Binding Sites Conformational transition Ethylmaleimide - pharmacology Guanosine Triphosphate - pharmacology Horses Molecular Conformation preincubation Receptors, Serotonin - drug effects Receptors, Serotonin - metabolism Serotonin Serotonin - metabolism Synaptosomes - metabolism Time Factors Tritium
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Zusammenfassung:	Binding of [ 3H]5-HT was studied wuing purified neuronal membranes isolted frrom horse brain striatum. It is shown that the preincubation of these membranes with an agonist (5-HT, bufotenin) followed by washibng, modifined the binding of [ 3H]5-GT from a low affinity to a high affinity constant. This effect was blocked by previous pretreatment of the membranes with a sulfhydryl group reagent (N-ethylmaleimide) and was reversed by guanoside 5′-triphosphate.
ISSN:	0014-2999 1879-0712
DOI:	10.1016/0014-2999(80)90218-6