Identification of 9-O-acetyl-N-acetylneuraminic acid on the surface of BALB/c mouse erythrocytes

Identification of 9-O-acetyl-N-acetylneuraminic acid on the surface of BALB/c mouse erythrocytes

For the first time 9-O-acetyl-N-acetylneuraminic acid has been unequivocally identified as the almost exclusive sialic acid of BALB/c mouse erythrocytes by gas-liquid chromatography/mass spectrometry. In human erythrocytes which were processed simultaneously N-acetylneuraminic acid could be identifi...

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Veröffentlicht in:Biochemical and biophysical research communications 1980-05, Vol.94 (2), p.567-572
Hauptverfasser: Reuter, Gerd, Vliegenthart, Johannes F.G., Wember, Margret, Schauer, Roland, Howard, Russell J.
Format: Artikel
Sprache:eng
Schlagworte:
Acetylation
Animals
Chromatography, Thin Layer
Erythrocyte Aging
Erythrocyte Membrane - analysis
Erythrocytes - analysis
Gas Chromatography-Mass Spectrometry
Humans
Mice
Mice, Inbred BALB C
Sialic Acids - blood
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Beschreibung
Zusammenfassung:For the first time 9-O-acetyl-N-acetylneuraminic acid has been unequivocally identified as the almost exclusive sialic acid of BALB/c mouse erythrocytes by gas-liquid chromatography/mass spectrometry. In human erythrocytes which were processed simultaneously N-acetylneuraminic acid could be identified as the only sialic acid. In 10 10 human erythrocytes 350 nmoles of sialic acid were found and in the same number of mouse erythrocytes 440 nmoles.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(80)91269-3