Effect of calcium on cyclic nucleotide phosphodiesterase in parathyroid tissue

The hydrolysis of cyclic AMP and cyclic GMP by homogenates of normal bovine parathyroid gland and human parathyroid adenomas was decreased by EGTA. When supernatants were chromatographed on DEAE-cellulose it was found that sheep brain calmodulin in the presence of calcium stimulated cyclic AMP and c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1980-06, Vol.94 (3), p.763-768
Hauptverfasser:	Willgoss, D., Jacobi, J.M., de Jersey, J., Bartley, P.C., Lloyd, H.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	3',5'-Cyclic-AMP Phosphodiesterases - isolation & purification 3',5'-Cyclic-AMP Phosphodiesterases - metabolism 3',5'-Cyclic-GMP Phosphodiesterases - isolation & purification 3',5'-Cyclic-GMP Phosphodiesterases - metabolism Animals Calcium - pharmacology Cattle Egtazic Acid - pharmacology Parathyroid Glands - drug effects Parathyroid Glands - enzymology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The hydrolysis of cyclic AMP and cyclic GMP by homogenates of normal bovine parathyroid gland and human parathyroid adenomas was decreased by EGTA. When supernatants were chromatographed on DEAE-cellulose it was found that sheep brain calmodulin in the presence of calcium stimulated cyclic AMP and cyclic GMP phosphodiesterase activity. The response to calmodulin in two human parathyroid adenomas was less than that in normal bovine parathyroid. Calmodulin was detected in heat-treated supernatants of 11 parathyroid adenomas by its ability to activate calmodulin-free sheep brain phosphodiesterase. The results suggest a role for calcium in the hydrolysis of cyclic nucleotides in parathyroid tissue.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(80)91300-5