Amboceptor Titration as a Statistically Controlled Assay

The relative potencies of amboceptors may be determined with data from experiments planned in factorial design and evaluated through the analysis of variance. Dose-response lines are calculated from the percentages of lysis obtained in the presence of a constant amount of complement (1.2 C'H50 units) at three different levels of the unknown and of a standard amboceptor preparation in the range of the supplementary zone. The method is illustrated with data from a typical assay and the results obtained with two more amboceptors are briefly reported.