Enzymatic biosynthesis of cephalexin

Enzymatic biosynthesis of cephalexin

The reaction kinetics of the enzymatic of cephalexin from 7‐aminodea‐cetoxy cephalosporanic acid and phenylglycine methylester was studied using the synthesizing enzyme obtained from Xanthomonas citri. The activation energy, Km value for 7‐aminodeacetoxy cephalosporanic acid and phenylglycine methyl...

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Veröffentlicht in:Biotechnology and bioengineering 1980-06, Vol.22 (6), p.1237-1247
Hauptverfasser: Rhee, D. K., Lee, S. B., Rhee, J. S., Ryu, Dewey D. Y., Hospodka, J.
Format: Artikel
Sprache:eng
Schlagworte:
Amidohydrolases - metabolism
Benzoates - pharmacology
Cephalexin - biosynthesis
Enzyme Induction
Kinetics
Oxygen - pharmacology
Penicillin Amidase - biosynthesis
Penicillin Amidase - metabolism
Phenylacetates - pharmacology
Xanthomonas - enzymology
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Zusammenfassung:The reaction kinetics of the enzymatic of cephalexin from 7‐aminodea‐cetoxy cephalosporanic acid and phenylglycine methylester was studied using the synthesizing enzyme obtained from Xanthomonas citri. The activation energy, Km value for 7‐aminodeacetoxy cephalosporanic acid and phenylglycine methylester, and Ki value for phenylglycine methylester were determined as 8.63 kcal/mol, 3.7mM, 14.5mM, and 70mM, respectively. The enzyme was found to be constitutive and susceptible to deactivation.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260220610