PARTIAL CHARACTERIZATION OF SOLUBLE ACETYLCHOLINESTERASE ISOENZYMES OF THE RAT BRAIN

PARTIAL CHARACTERIZATION OF SOLUBLE ACETYLCHOLINESTERASE ISOENZYMES OF THE RAT BRAIN

(1) The 105,000 g supernatant fluid obtained from rat brain was separated by agar‐gel electrophoresis. (2) Three isoenzymes, capable of hydrolysing acetylthiocholine, one of them also hydrolysing butyrylthiocholine, were detected. (3) The pH optima and Km for hydrolysis of acetyl‐ and butyrylthiocho...

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Veröffentlicht in:Journal of neurochemistry 1971-09, Vol.18 (9), p.1609-1614
Hauptverfasser: Bajgar, J., ŽIžkovský, V.
Format: Artikel
Sprache:eng
Schlagworte:
Acetylcholine
Acetylcholinesterase - analysis
Animals
Brain - enzymology
Butyrates
Choline
Electrophoresis
Female
Hydrogen-Ion Concentration
Hydrolysis
Isoenzymes - analysis
Kinetics
Rats
Solubility
Sulfides
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Zusammenfassung:(1) The 105,000 g supernatant fluid obtained from rat brain was separated by agar‐gel electrophoresis. (2) Three isoenzymes, capable of hydrolysing acetylthiocholine, one of them also hydrolysing butyrylthiocholine, were detected. (3) The pH optima and Km for hydrolysis of acetyl‐ and butyrylthiocholine by the supernatant fluid were determined. (4) After extraction of acetylcholinesterase isoenzymes from the gel, individual isoenzymes were characterized by pH optima and Km values. (5) Two of the enzymes were characterized as acetylcholinesterase (EC 3.1.1.7) and one as butyrylcholinesterase (EC 3.1.1.8).
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.1971.tb03734.x