A newly-designed positioning system for simultaneous indocyanine green and fluorescein anterior segment angiography of the albino rabbit

Simultaneous recording of vertical iris indocyanine green angiography (ICGA) and iris fluorescein sodium angiography (IFA) in albino rabbits. An easily adjusted control system was designed to position the CSLO scanning lens perpendicular to the surface of the iris. Twelve albino rabbits were randomly divided into 2 groups. Using 5 mg fluorescein sodium and 5 mg indocyanine green, iris angiograms (IA) of 6 albino rabbits were performed with application of the positioning system in Group A, and no positioning system in Group B. The time spending on vertical eye position and the effects of simultaneous IA were observed. With the use of the positioning system, the irises of rabbits quickly achieved the vertical site, averaging 37.50+/-8.17 s in Group A, and 408.33+/-58.79 s in Group B. The difference was statistically significant, and the time saved averaged 370.83 s. Compared with the other methods of single-dye angiography, simultaneous digital angiography provided 2 kinds of full, dynamic videos of iris vessels in all albino rabbits. The emergence time of IFA and ICGA was 5-9 s, with an average time for ICGA of 6.4 s, and for IFA of 6.5 s. Except for leakage, the simultaneous ICGA and IFA appearance of this vascular pattern was the same as the single IFA described previously. Simultaneous vertical IA with indocyanine green and fluorescein sodium is a potentially powerful technique that allows high-quality fluorescence imaging of the albino rabbit iris.