High-Throughput Method for Determining the Enantioselectivity of Enzyme-Catalyzed Hydroxylations Based on Mass Spectrometry

High-Throughput Method for Determining the Enantioselectivity of Enzyme-Catalyzed Hydroxylations Based on Mass Spectrometry

Up to speed: An accurate, sensitive, high‐throughput, and simple method for measuring the product ee value of enzyme‐catalyzed hydroxylations (see scheme) is based on the use of enantiopure or enantioenriched deuterated substrates and mass spectrometric detection.

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Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie International Edition 2010-07, Vol.49 (31), p.5278-5283
Hauptverfasser: Chen, Yongzheng, Tang, Weng Lin, Mou, Jie, Li, Zhi
Format: Artikel
Sprache:eng
Schlagworte:
Catalysis
enantioselectivity
enzyme catalysis
high-throughput screening
Humans
Hydroxylation
Kinetics
mass spectrometry
Mass Spectrometry - methods
Mixed Function Oxygenases - chemistry
Mixed Function Oxygenases - metabolism
Pseudomonas - chemistry
Pseudomonas - metabolism
Stereoisomerism
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Beschreibung
Zusammenfassung:Up to speed: An accurate, sensitive, high‐throughput, and simple method for measuring the product ee value of enzyme‐catalyzed hydroxylations (see scheme) is based on the use of enantiopure or enantioenriched deuterated substrates and mass spectrometric detection.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201001772