Molecular identification of Na+-H+ exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus

Molecular identification of Na+-H+ exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus

In the current study, reverse‐transcription polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full‐length putative Na+–H+ exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a prote...

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Veröffentlicht in:Journal of fish biology 2010-02, Vol.76 (2), p.415-426
Hauptverfasser: Edwards, S. L., Weakley, J. C., Diamanduros, A. W., Claiborne, J. B.
Format: Artikel
Sprache:eng
Schlagworte:
acid-base regulation
Amino Acid Sequence
Animals
Fundulidae - classification
Fundulidae - metabolism
Fundulus heteroclitus
gills
Gills - metabolism
In Situ Hybridization
Marine
Molecular Sequence Data
Na+-H+ exchanger
Phylogeny
Sequence Alignment
Sodium-Hydrogen Exchangers - chemistry
Sodium-Hydrogen Exchangers - genetics
Sodium-Hydrogen Exchangers - metabolism
teleost
Teleostei
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Zusammenfassung:In the current study, reverse‐transcription polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full‐length putative Na+–H+ exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.
ISSN:0022-1112
1095-8649
DOI:10.1111/j.1095-8649.2009.02534.x