A new highly sensitive point of care screen for spontaneous bacterial peritonitis using the leukocyte esterase method

Background & Aims Urine reagent strips measuring leukocyte esterase activity have been studied to screen spontaneous bacterial peritonitis (SBP) but are insensitive. We calibrated a strip specifically for ascitic fluid to achieve high sensitivity in this diagnosis. Methods Experiments were condu...

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Veröffentlicht in:	Journal of hepatology 2010-09, Vol.53 (3), p.477-483
Hauptverfasser:	Mendler, Michel Henry, Agarwal, Arun, Trimzi, Mateen, Madrigal, Erika, Tsushima, Matthew, Joo, Elliot, Santiago, Marisela, Flores, Elissa, David, Gabriel, Workman, Adrienne, Runyon, Bruce
Format:	Artikel
Sprache:	eng
Schlagworte:	Abdomen Ascites Ascitic Fluid - enzymology Bacterial Infections - diagnosis Bacterial Infections - enzymology Bacterial Infections - etiology Biological and medical sciences Carboxylic Ester Hydrolases - analysis False Positive Reactions Female Gastroenterology and Hepatology Gastroenterology. Liver. Pancreas. Abdomen Humans Hypertension, Portal - complications Leukocyte esterase Liver Cirrhosis - complications Male Medical sciences Middle Aged Neutrophils - enzymology Other diseases. Semiology Peritonitis - diagnosis Peritonitis - enzymology Peritonitis - etiology Point of care testing Point-of-Care Systems - statistics & numerical data Reagent Strips Screen Sensitivity and Specificity Spontaneous bacterial peritonitis
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Zusammenfassung:	Background & Aims Urine reagent strips measuring leukocyte esterase activity have been studied to screen spontaneous bacterial peritonitis (SBP) but are insensitive. We calibrated a strip specifically for ascitic fluid to achieve high sensitivity in this diagnosis. Methods Experiments were conducted on ascitic fluid from patients with cirrhosis. Samples with SBP were diluted with native acellular ascitic fluid to achieve PMN counts below, above, and close to the diagnostic threshold of 250 PMN/μl. A model of SBP was created by spiking negative ascitic fluid samples (
ISSN:	0168-8278 1600-0641
DOI:	10.1016/j.jhep.2010.04.011