A Method for Micro-Scale Isolation of Sulfated Glycopeptide from Tissue

A Method for Micro-Scale Isolation of Sulfated Glycopeptide from Tissue

Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose...

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Veröffentlicht in:The Tohoku Journal of Experimental Medicine 1979, Vol.129(3), pp.225-231
Hauptverfasser: ENDO, MASAHIKO, YOSIZAWA, ZENSAKU
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Electrophoresis
Female
Glycopeptides - isolation & purification
Microchemistry - methods
rabbit uterus
Rabbits
sulfated glycopeptide
Sulfates - isolation & purification
Uterus - analysis
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Beschreibung
Zusammenfassung:Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in a high yield from estrogen-treated rabbit uterus.
ISSN:0040-8727
1349-3329
DOI:10.1620/tjem.129.225