Kinetic analysis of RNA interference for lamin A/C in HeLa cells

We kinetically analyzed RNA interference （RNAi） for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA （siRNA） by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drugresponse system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.