Synthesis of Aminooxy and N-Alkylaminooxy Amines for Use in Bioconjugation

Five Boc-protected aminooxy and N-alkylaminooxy amines have been synthesized in 60−95% overall yield using a common synthetic strategy from readily available two- and three-carbon Cbz-protected amino alcohols. The amines can be linked to biomolecules via amide formation and incorporated directly into peptoids via submonomer synthesis. Subsequent deprotection of the aminooxy and N-alkylaminooxy groups enables conjugation with desired target molecules via established chemoselective ligation methods. The range of derivatives synthesized allows different distances to be established between the conjugated molecules.