Identification and characterization of uncoupling protein in heart and muscle mitochondria of canary birds

An uncoupling protein (cUCP) was identified in heart and skeletal muscle mitochondria of canary birds. cUCP was immunodetected using polyclonal antibodies raised against murine UCP2. Its molecular mass was similar to those of mammalian UCPs (32 kDa). The activity of cUCP was stimulated by palmitic a...

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Veröffentlicht in:	Journal of bioenergetics and biomembranes 2010-08, Vol.42 (4), p.345-353
Hauptverfasser:	Slocinska, Malgorzata B, Almsherqi, Zakaria Ali Moh, Sluse, Francis E, Navet, Rachel, Deng, Yuru
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Animal Anatomy Animal Biochemistry Animals Biochemistry Bioorganic Chemistry Birds Canaries - metabolism Canary birds Chemistry Chemistry and Materials Science heart Histology Ion Channels - analysis Ion Channels - metabolism Mitochondria Mitochondria, Heart - metabolism Mitochondria, Muscle - metabolism Mitochondrial Proteins - analysis Mitochondrial Proteins - metabolism Morphology Muscle, Skeletal - metabolism Muscles Organic Chemistry Oxidation Proteins Respiration skeletal muscle Uncoupling protein Uncoupling Protein 1
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Zusammenfassung:	An uncoupling protein (cUCP) was identified in heart and skeletal muscle mitochondria of canary birds. cUCP was immunodetected using polyclonal antibodies raised against murine UCP2. Its molecular mass was similar to those of mammalian UCPs (32 kDa). The activity of cUCP was stimulated by palmitic acid (PA) and inhibited by GTP mainly in state 3 respiration. Additions of PA augmented state 4 respiration and lowered the ADP/O ratio. Thus, the activity of cUCP diverted energy from oxidative phosphorylation in state 3 respiration. cUCP in heart and skeletal muscles of canary birds might have implications in thermogenesis as well as protection against free radical production.
ISSN:	0145-479X 1573-6881
DOI:	10.1007/s10863-010-9303-x