Phase and amplitude contrast in electron microscopy of stained biological objects

For biological objects negatively stained with heavy atom material, electron microscope images show best contrast for image detail on the scale of 10–20 Å when a small objective aperture is used. In images taken under the optimum phase contrast imaging conditions of Scherzer, the required image deta...

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Veröffentlicht in:Ultramicroscopy 1979, Vol.4 (4), p.419-427
Hauptverfasser: Cowley, J.M., Bridges, R.E.
Format: Artikel
Sprache:eng
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Zusammenfassung:For biological objects negatively stained with heavy atom material, electron microscope images show best contrast for image detail on the scale of 10–20 Å when a small objective aperture is used. In images taken under the optimum phase contrast imaging conditions of Scherzer, the required image detail is lost in unwanted noise. Both of these conditions may be described in terms of phase contrast imaging for a thin phase object. Calculations of image intensities and noise are reported for a model object consisting of heavy and light atoms randomly distributed to simulate a negatively stained protein molecule. The results are consistent with experimental observations.
ISSN:0304-3991
1879-2723
DOI:10.1016/S0304-3991(79)80019-4