Charcoal assay for intrinsic factor using membrane filtration

Charcoal assay for intrinsic factor using membrane filtration

A modification of the albumin-coated charcoal method for the assay of intrinsic factor and other vitamin B 12 binders is described. It replaces centrifugation with ultrafiltration under reduced pressure through small pore membranes. The resulting method is quicker, and more accurate. In addition, it...

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Veröffentlicht in:Analytical biochemistry 1971-06, Vol.41 (2), p.402-407
Hauptverfasser: Bloomfield, F.J., Scott, John M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Centrifugation
Charcoal
Cobalt Isotopes
Filtration
Gastric Juice - analysis
Humans
Immune Sera
Intrinsic Factor - analysis
Kinetics
Methods
Serum Albumin, Bovine
Time Factors
Vitamin B 12
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Beschreibung
Zusammenfassung:A modification of the albumin-coated charcoal method for the assay of intrinsic factor and other vitamin B 12 binders is described. It replaces centrifugation with ultrafiltration under reduced pressure through small pore membranes. The resulting method is quicker, and more accurate. In addition, it makes it possible to study the kinetic of agents thought to split vitamin B 12 macromolecular complexes by separating the free from the bound vitamin in seconds.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(71)90158-8