CHARACTERIZATION OF METHANOL EXTRACTION RESIDUE (MER): FROM BACILLUS CALMETTE-GUÉRIN (BCG)

CHARACTERIZATION OF METHANOL EXTRACTION RESIDUE (MER): FROM BACILLUS CALMETTE-GUÉRIN (BCG)

Analysis of methanol extraction residue (MER) from Bacillus CALMETTE-GUËRI(BCG) was carried out to determine some specific chemical compositional characteristics. Samples of MER were found to contain approximately 40% protein and/or peptide, 3% soluble lipids, 17% bound lipids, 8% elemental nitrogen...

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Veröffentlicht in:Journal of antibiotics 1979, Vol.32(10), pp.1011-1015
Hauptverfasser: SHAIKH, BADARUDDIN, ZIELINSKI, WALTER L., Jr, DOUROS, JOHN D.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - analysis
Bacterial Proteins - analysis
Chemical Phenomena
Chemistry
Lipids - analysis
Methanol
Mycobacterium bovis - analysis
Mycolic Acids - analysis
Nitrogen - analysis
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Zusammenfassung:Analysis of methanol extraction residue (MER) from Bacillus CALMETTE-GUËRI(BCG) was carried out to determine some specific chemical compositional characteristics. Samples of MER were found to contain approximately 40% protein and/or peptide, 3% soluble lipids, 17% bound lipids, 8% elemental nitrogen, and less than 2% mycolic acids. Amino acid analysis showed the presence of alanine, glycine and glutamic acid as the major amino acids. The data are reported in terms of the range found for each constituent over the samples analyzed. Somewhat consistent results were obtained between different MER preparations, but notable compositional variations were observed in samples of MER suspensions.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.32.1011