Isolation of granules containing adipokinetic hormone from locust corpora cardiaca by differential centrifugation

Using differential centrifugation a fraction rich in AKH has been isolated from the glandular lobes of the corpus cardiacum of Schistocerca. This fraction, obtained by centrifugation at 30,000 g in 0.44 M sucrose, when examined under the electron microscope, was seen to consist of electron dense granules of size range 100–600 nm. These granules are identical with the neurosecretory granules seen in the intact glandular lobe cells.