Organization of the migrating chick epiblast edge: Attachment sites, cytoskeleton, and early developmental changes

The radial expansion of the chick extraembryonic epiblast on the inner side of the vitelline membrane in yolk sac formation provides a useful system for study of adhesion and migration of an epithelial cell sheet. A band of specialized cells at the epiblast edge adheres by its dorsal side to the overlying vitelline membrane. The attached edge was examined by scanning electron microscopy. The attachment region (av 0.06 mm wide) extends from the advancing edge to a transitional ridge. The ridge appears to be an area of adhesion and de-adhesion. The attached surface is smooth with small surface projections and filopodia. These become more numerous and prominent with cold treatment. Epiblast cells display a filopodial/lamellipodial mode of migration in vivo and in vitro. The distribution of 4- to 7-nm microfilaments in edge cells is examined using transmission electron microscopy of whole cells. Decoration with heavy meromyosin shows that these components of the cytoskeleton contain actin. Treatment of intact blastoderms and dissociated edge cells with cytochalasin B and cold suggests that microfilaments rather than microtubules are primarily responsible for edge cell morphology. Early blastoderm cells which have not initiated migration respond to cytochalasin B, cold, and colcemid in the same way as migrating edge cells. This suggests that the differentiative change that produces the rapidly migrating edge cells does not involve a shift in the relative contribution of microtubules and microfilaments to the cytoskeleton.