Direct tissue isoelectric focusing in agarose

Direct tissue isoelectric focusing in agarose

We are isoelectric focusing small amounts of tissues placed directly on electroendosmosis-free agarose gels instead of using the saline extracts of tissue homogenates. More numerous proteins are extracted during isoelectric focusing of the solid tissues than present in the saline extracts of the sam...

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Veröffentlicht in:Journal of immunological methods 1979, Vol.29 (1), p.97-100
Hauptverfasser: Saravis, C.A., O'Brien, M., Zamcheck, N.
Format: Artikel
Sprache:eng
Schlagworte:
Adenocarcinoma
Colonic Neoplasms
Histological Techniques
Humans
Isoelectric Focusing - methods
Neoplasm Proteins - isolation & purification
Sepharose
Time Factors
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Zusammenfassung:We are isoelectric focusing small amounts of tissues placed directly on electroendosmosis-free agarose gels instead of using the saline extracts of tissue homogenates. More numerous proteins are extracted during isoelectric focusing of the solid tissues than present in the saline extracts of the same tissues.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(79)90131-5