A radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes
A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Aver...
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| Veröffentlicht in: | Journal of immunological methods 1979-01, Vol.29 (3), p.253-262 |
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| container_title | Journal of immunological methods |
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| creator | Del Villano, Bert C. Tischfield, Jay A. |
| description | A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using
Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Average values for SOD-1 in lysates of erythrocytes from normal individuals is 854 ± 100 ng/mg hemoglobin and that of patients with trisomy-21 is 1313 ± 110 ng/mg hemoglobin. |
| doi_str_mv | 10.1016/0022-1759(79)90313-2 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Journal of immunological methods, 1979-01, Vol.29 (3), p.253-262 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74747034 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies Antibody Specificity Binding, Competitive Down Syndrome - immunology Electrophoresis, Polyacrylamide Gel Epitopes Erythrocytes - enzymology Humans Immune Sera - pharmacology Immunoenzyme Techniques Radioimmunoassay Superoxide Dismutase - analysis |
| title | A radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes |
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