A radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes

A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Aver...

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Veröffentlicht in:	Journal of immunological methods 1979-01, Vol.29 (3), p.253-262
Hauptverfasser:	Del Villano, Bert C., Tischfield, Jay A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibody Specificity Binding, Competitive Down Syndrome - immunology Electrophoresis, Polyacrylamide Gel Epitopes Erythrocytes - enzymology Humans Immune Sera - pharmacology Immunoenzyme Techniques Radioimmunoassay Superoxide Dismutase - analysis
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Zusammenfassung:	A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Average values for SOD-1 in lysates of erythrocytes from normal individuals is 854 ± 100 ng/mg hemoglobin and that of patients with trisomy-21 is 1313 ± 110 ng/mg hemoglobin.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(79)90313-2