Isolation and structure determination of enzymatically formed styrene oxide glutathione conjugates

Isolation and structure determination of enzymatically formed styrene oxide glutathione conjugates

When styrene oxide was incubated with glutathione in the presence of rat liver cytosolic fraction, two conjugates were formed. Structural investigation by mass spectrometry (MS), proton magnetic resonance (PMR) analysis and chemical fragmentation showed the presence of two positional isomers, namely...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemico-biological interactions 1979-10, Vol.27 (2), p.313-321
Hauptverfasser: Pachecka, J., Gariboldi, P., Cantoni, L., Belvedere, G., Mussini, E., Salmona, M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cytosol - metabolism
Epoxy Compounds - metabolism
Ethers, Cyclic - metabolism
Glutathione - analogs & derivatives
Glutathione - metabolism
Liver - metabolism
Magnetic Resonance Spectroscopy
Male
Rats
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:When styrene oxide was incubated with glutathione in the presence of rat liver cytosolic fraction, two conjugates were formed. Structural investigation by mass spectrometry (MS), proton magnetic resonance (PMR) analysis and chemical fragmentation showed the presence of two positional isomers, namely S-(1-phenyl-2-hydroxyethyl)glutathione and S-(2-phenyl-2-hydroxyethyl)glutathione in a ratio of approx. 60 : 40.
ISSN:0009-2797
1872-7786
DOI:10.1016/0009-2797(79)90134-0