Viral transformation of chick myogenic cells: The relationship between differentiation and the expression of the SRC gene
When chick embryo presumptive muscle cells are transformed at 35 °C with a temperature-sensitive mutant of Rous sarcoma virus, tsLA29, they do not undergo myogenic differentiation. When these cultures are shifted to 41 °C the cells revert to a phenotypically normal state and fuse into myotubes. The...
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Veröffentlicht in: | Experimental cell research 1979-10, Vol.123 (1), p.95-105 |
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Sprache: | eng |
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Zusammenfassung: | When chick embryo presumptive muscle cells are transformed at 35 °C with a temperature-sensitive mutant of Rous sarcoma virus,
tsLA29, they do not undergo myogenic differentiation. When these cultures are shifted to 41 °C the cells revert to a phenotypically normal state and fuse into myotubes. The synthesis of myosin, the appearance of myosin mRNA active in vitro, and the synthesis of acetylcholinesterase (AChE) were all activated following a shift from 35 °C to 41 °C. The activation of myosin synthesis also occurred in cultures prevented from fusing by calcium deprivation. After myosin synthesis had been initiated at 41 °C, however, it could not be suppressed by shifting the cultures back to 35 °C. [
3H]Thymidine labeling and autoradiography demonstrated that DNA synthesis in
tsLA29-infected myoblasts ceased within 24 h after the shift to 41°C. A kinetic analysis of the withdrawal of these cells from the cell cycle indicates that at least a fraction of the cells do not need to traverse a complete cell cycle prior to terminal differentiation. |
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ISSN: | 0014-4827 1090-2422 |
DOI: | 10.1016/0014-4827(79)90425-7 |