New methods for the purification of vertebrate vitellogenin

Two procedures are described for the isolation of vitellogenin from plasma. Vitellogenin can be selectively precipitated from the plasma of estrogen-treated animals by Mg 2+ in the presence of EDTA. The vitellogenin thus obtained is >99% pure and is relatively undegraded. In the second procedure,...

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Veröffentlicht in:	Anal. Biochem.; (United States) 1979-08, Vol.97 (1), p.145-152
Hauptverfasser:	Wiley, H.Steven, Opresko, Lee, Wallace, Robin A.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques AMINO ACIDS Animals ATOMIC IONS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES CALCIUM IONS CARBOHYDRATES CARBOXYLIC ACIDS CELLULOSE CHARGED PARTICLES CHELATING AGENTS Chemical Precipitation CHEMICAL PREPARATION CHROMATOGRAPHY Chromatography, DEAE-Cellulose DATA DATA FORMS DAYS LIVING RADIOISOTOPES EDTA EFFICIENCY Electrophoresis, Polyacrylamide Gel ESTERS ESTROGENS Estrogens - administration & dosage EXPERIMENTAL DATA EXTRACTION CHROMATOGRAPHY Female HORMONES IMPURITIES INFORMATION IONS ISOLATED VALUES ISOTOPE APPLICATIONS ISOTOPES LIGHT NUCLEI LIPIDS LIPOPROTEINS Lipoproteins - isolation & purification MAGNESIUM IONS Methods NUCLEI NUMERICAL DATA ODD-ODD NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC PHOSPHORUS COMPOUNDS PHOSPHOLIPIDS PHOSPHORUS 32 PHOSPHORUS ISOTOPES POLYSACCHARIDES PRECIPITATION PROTEINS RADIOISOTOPES SACCHARIDES SEPARATION PROCESSES STEROID HORMONES SYNTHESIS TRACER TECHNIQUES Vitellogenins - isolation & purification Xenopus - blood
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Beschreibung
Zusammenfassung:	Two procedures are described for the isolation of vitellogenin from plasma. Vitellogenin can be selectively precipitated from the plasma of estrogen-treated animals by Mg 2+ in the presence of EDTA. The vitellogenin thus obtained is >99% pure and is relatively undegraded. In the second procedure, vitellogenin can also be isolated by DEAE-cellulose chromatography, resulting in preparations that contain
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(79)90338-5