Hybridization method for quantitation of replicating polyoma DNA sequences

Hybridization method for quantitation of replicating polyoma DNA sequences

Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975, J. Mol. Biol. 98, 503–517). The filters were then used in hybr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical biochemistry 1979-07, Vol.96 (2), p.378-383
Hauptverfasser: Spaeren, Unni, Waldeck, Waldemar, Eliasson, Rolf
Format: Artikel
Sprache:eng
Schlagworte:
Carbon Radioisotopes
DNA Replication
DNA Restriction Enzymes
DNA, Viral - analysis
Methods
Nucleic Acid Hybridization
Polyomavirus - analysis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975, J. Mol. Biol. 98, 503–517). The filters were then used in hybridization experiments to localize the isotope in different parts of the polyoma genome after in vitro incorporation of labeled deoxyribonucleoside triphosphates into the DNA.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(79)90595-5