Lethal effects of 32P decay on transfecting activity of Bacillus subtilis phage φe DNA
Disintegration of 32P present in the DNA of Bacillus subtilis phage φe (a phage containing double-strand DNA) results in the loss of viability of intact phage as well as transfecting activity of isolated DNA. Only 1 12 of the 32P disintegrations per phage DNA equivalent inactivates the intact phage...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1979-07, Vol.96 (1), p.270-273 |
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| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | Disintegration of
32P present in the DNA of
Bacillus subtilis phage φe (a phage containing double-strand DNA) results in the loss of viability of intact phage as well as transfecting activity of isolated DNA. Only
1
12
of the
32P disintegrations per phage DNA equivalent inactivates the intact phage while nearly every disintegration inactivates the transfecting DNA. This result provides evidence for a single-strand intermediate in the transfection of
B. subtilis by φe DNA. |
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| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1016/0042-6822(79)90191-0 |