Calibration mixture for estimation of peptide molecular weight by exclusion chromatography
A procedure is described for the rapid preparation of a partial cyanogen bromide digest of horse heart cytochrome c. This digest serves as a calibration mixture for estimation of peptide molecular weight in the range 500 to 20,000 by exelusion chromatography in denaturing solvents. The elution profi...
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| Veröffentlicht in: | Analytical biochemistry 1979-04, Vol.94 (1), p.228-230 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | A procedure is described for the rapid preparation of a partial cyanogen bromide digest of horse heart cytochrome
c. This digest serves as a calibration mixture for estimation of peptide molecular weight in the range 500 to 20,000 by exelusion chromatography in denaturing solvents. The elution profile of the calibration mixture from Sephadex G-50 equilibrated and developed with 10% formic acid is described by the relationship log
M = 4.79 − 0.42 (
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| ISSN: | 0003-2697 1096-0309 |
| DOI: | 10.1016/0003-2697(79)90813-3 |