Screening of anti-cancer material on kinds of sponges and gorgonian against the L1210 cell line

The long history of the evolution of marine organisms has made these organisms as a source of high molecular diversity. In searching a new anti cancer drugs, these organisms have become an intense objects of research. Sponges and gorgonian were collected from Jepara and Labuan Bajo-Flores Waters from the depth of 1-3 m and 3-38 m respectively by Skin and SCUBA diving. The samples were extracted with methanol. The extracts were assayed against cancer cell (L1210 cell line) with tested concentration ranging from 0, 1, 5 and 10 ppm. The cell line was cultured in complete RPMI media and growth inhibition was measured with direct counting. Methanol extract of sponges and gorgonian yielded approximately from 0.55 - 24.7% of dry weight or 0.36 - 7.34% of wet basis. All methanol extracts of the samples could inhibit the growth of L 1210 cell line and is potential for further purification. Methanol extracts from 3 species of sponges (Xestospongia sp and Phyllospongia sp and UP8) and one ethyl acetate fraction of I. Hippuris had IC-50 value < 3 mg/mL. Methanol extract of 8 species of sponges (Agelas nakamurai, Ircina ramosa, A06. Phyllospongia lamellosa. Phyllospongia sp. UP9, Calispongla sp and Fascaplynopsis sp) had IC-50 < 5 mg/mL, methanol extract of 5 species of sponges (Hyrtios erecta. Xestospongia sp. Cladocroce sp, Oceanapia cf. Amboiensis and Haliclona sp.) and one water extract of I. hippuris had IC-50 < 10 mg mL. Methanol extracts of one species of sponge (Xestospongla sp) had IC-50 > 10 mg/mL.Original Abstract: Potensi biota laut sebagai sumber obat anti kanker menjadi objek penelitian penting dalam tahun-tahun terakhir. Bahan yang dipergunakan dalam penelitian ini adalah sponge dan gorgonian yang dikoleksi dari Perairan Jepara pada kedalaman 1-3 m dan di perairan Labuhan Bajo, Flores pada kedalaman 3-38 m. Sampling dilakukan dengan Skin diving dan SCUBA diving. Sampel diekstrak dengan metanol, selanjutnya ekstrak diujikan terhadap sel kanker leukemia (L1210 cell line) dengan konsentrasi 0, 1. 5 dan 10 ppm. Uji dilakukan pada media RPMl lengkap dan penghitungan daya hambat dilakukan dengan metoda direct counting. Ekstrak yang diperoleh berkisar antara 0.55 - 24,7% dari berat kering atau 0.36-7.34% dari berat basahnya. Seluruh ekstrak dari sampel-sampel mampu menghambat pertumbuhan L1210 cell line dan layak untuk pemurnian lanjut. Ekstrak metanol tiga jenis sponge (Xestospongia sp dan Phyllospongia sp dan UP8) dan fraksi etil asetat dari ekstrak gorgonian