Toxicity test of Gorgonian Isis hippuris extract on Nauplius Artemia salina

The objective of this study is to investigate Gorgonian Isis hippuris extract toxicity to Nauplius Artemia salina. This was done by calculating LC50-24 h and followed by identifying its compound. This study was done at Exploration and Biotechnology Laboratorium, Marine Science Department, Diponegoro University, Teluk Awur, Jepara. The results showed that of 12 ethyl acetate fractions resulted from open column chromatography, 5 of them were toxic. Based on probit analysis it was found that fraction 9 was the best one for giving LC50-24 h 16.98 ppm. Based on GC/MS results there are several compound found i.e. hydrocarbon, lipid acid (napthalene, xylane, phenylacetonitrile, 1.2 benzenedicarboxylic) and phenol derived compound.Original Abstract: Tujuan penelitian ini adalah untuk mengetahui aktivitas toksisitas ekstrak Gorgonian isis hippuris terhadap Nauplius Artemia salina, mengetahui LC50-24 jam fraksi toksik dan mengidentifikasi senyawa yang berperan dalam toksisitas. Penelitian dilaksanakan di Laboratorium Ekplorasi dan Bioteknologi IImu Kelautan UNDIP, Teluk Awur Jepara. Hasil penelitian menunjukkan perlakuan ekstrak Gorgonian I. hippuris fraksi etil asetat terhadap Nauplius A. salina dari 12 fraksi KKT diperoleh 5 fraksi KKT yang toksik. Berdasarkan analisa probit diketahui fraksi KKT 9 memiliki aktivitas toksik terbaik dengan nilai LC50-24 jam sebesar 16,98 ppm. Hasil identifikasi dengan GC/MS diperoleh golongan senyawa-senyawa hidrokarbon dan asam lemak yaitu napthalene, xylane, phenylacetonitrile, 1,2 benzenedicarboxylic dan senyawa turunan phenol.