Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica)

The widespread use of medicinal plants for health purposes has increased dramatically due to their great importance to public health. In this study, the levels of phenolic, flavonoid, b-carotene and lycopene compounds of Anastatica hierochuntica and Hyphaene thebaica were determined. The plant extracts were evaluated for their antioxidant activities using various antioxidant methodologies: (i) scavenging of free radicals using 2, 2-diphenyl-1-picrylhydrazyl, (ii) metal ion chelating capacity, and (iii) scavenging of superoxide anion radical. The antimicrobial activity of both plant extracts was evaluated against a panel of microorganisms using the agar disc diffusion method. The total phenolic content (51.97 and 64.9 mg/g dry weight in A. hierochuntica and H. thebaica, respectively) was significantly (p < 0.05) different. The antioxidant activity increased with an increase in concentration. The plant extracts were more active against Gram-positive bacteria than Gram-negative bacteria. Also, the antimicrobial activity of H. thebaica was higher than that of A. hierochuntica methanolic extracts. This study reveals that the consumption of these plants would exert several beneficial effects by virtue of their antioxidant and antimicrobial activities.Original Abstract: El extensivo uso de plantas medicinales con fines medicos ha aumentado dramaticamente debido en gran parte a la importancia que tienen en la salud publica. En este estudio, los niveles de compuestos fenolicos, flavonoides, b-caroteno y licopeno de Anastatica hierochuntica y Hyphaene thebaica fueron determinados. Los extractos de plantas fueron evaluados mediante su actividad antioxidante usando varias metodologias: (i) captacion de radicales libres usando 2,2-difenil-1-picrilhidrazina, (ii) capacidad quelatante de iones metalicos, y (iii) captacion de radicales superoxidos. La actividad antimicrobiana de ambos extractos de planta fue evaluada mediante un panel de microorganismos usando el metodo de difusion en disco de agar. El contenido total de fenoles (51.97 y 64.9 mg/g peso seco en A. hierochuntica y H. thebaica, respectivamente) fue significativamente diferente (p < 0.05). La actividad antioxidante incremento con un aumento de la concentracion. Los extractos de planta fueron mas activos contra bacterias Gram-positiva que contra bacterias Gram-negativas. Tambien, la actividad antimicrobiana de los extractos metanolicos de H. thebaica fue mayor que la de los extractos metanolicos de A. hierochu