A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes
Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2...
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Veröffentlicht in: | Experimental parasitology 2010-06, Vol.125 (2), p.179-183 |
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Sprache: | eng |
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Zusammenfassung: | Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the
SPECT2 gene of
Plasmodium berghei and the
cytochrome oxidase subunit I gene of
Dirofilaria immitis in mosquitoes
. This technique could detect as few as 100
P. berghei-infected red blood cell-equivalents or one
D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs. |
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ISSN: | 0014-4894 1090-2449 |
DOI: | 10.1016/j.exppara.2009.12.023 |