A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes

Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2...

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Veröffentlicht in:Experimental parasitology 2010-06, Vol.125 (2), p.179-183
Hauptverfasser: Aonuma, Hiroka, Yoshimura, Aya, Kobayashi, Tomomi, Okado, Kiyoshi, Badolo, Athanase, Nelson, Bryce, Kanuka, Hirotaka, Fukumoto, Shinya
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Sprache:eng
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Zusammenfassung:Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2 gene of Plasmodium berghei and the cytochrome oxidase subunit I gene of Dirofilaria immitis in mosquitoes . This technique could detect as few as 100 P. berghei-infected red blood cell-equivalents or one D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2009.12.023