A comparison of methods for obtaining high yields of pure immunoglobulin from severely haemolysed plasma

Ammonium sulphate, DEAE ion exchange chromatography, and polyethylene glycol (PEG) were compared for their ability to isolate and purify large quantities of immunoglobuins from severely haemolysed plasma. PEG, which produced the highest yield and purity, proved to be the only method which completely...

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Veröffentlicht in:	Journal of immunological methods 1979, Vol.26 (3), p.213-222
Hauptverfasser:	Carter, Robert J., Boyd, Nigel D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Sulfate Chemical Precipitation Chromatography, Ion Exchange - methods Hemolysis Humans Immunoglobulins - isolation & purification Immunologic Techniques Polyethylene Glycols
Online-Zugang:	Volltext
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Zusammenfassung:	Ammonium sulphate, DEAE ion exchange chromatography, and polyethylene glycol (PEG) were compared for their ability to isolate and purify large quantities of immunoglobuins from severely haemolysed plasma. PEG, which produced the highest yield and purity, proved to be the only method which completely removed the haemoglobin.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(79)90246-1