A new, convenient method for the rapid analysis of inorganic pyrophosphate

A new, convenient method for the rapid analysis of inorganic pyrophosphate

A new method for the rapid analysis of inorganic pyrophosphate ( PP i) which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5–50.0 nmol of PP i and is not affected by inorganic p...

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Veröffentlicht in:Analytical biochemistry 1979-04, Vol.94 (1), p.117-120
Hauptverfasser: Drake, Harold L., Goss, Neil H., Wood, Harland G.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphate - analysis
Catalysis
Chemical Phenomena
Chemistry
Diphosphates - analysis
Methods
Spectrophotometry
Sulfate Adenylyltransferase
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Zusammenfassung:A new method for the rapid analysis of inorganic pyrophosphate ( PP i) which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5–50.0 nmol of PP i and is not affected by inorganic phosphate. ATP and PP i can both be analyzed using this method.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(79)90800-5